COMPACT Publications

1 - Going beyond the liver: Progress and challenges of targeted delivery of siRNA therapeutics Cornelia Lorenzer, Mehrdad Dirin, Anna-Maria Winkler, Volker Baumann, JohannesWinkler
Journal of Controlled Release

2 - Carrier interactions with the biological barriers of the lung: Advanced in vitro models and challenges for pulmonary drug delivery Cristiane de Souza Carvalho, Nicole Daum, Claus-Michael Lehr
Advanced Drug Delivery Reviews

3 - Effects of cargo molecules on membrane perturbation caused by transportan10 based cell-penetrating peptides Luís Vasconcelos, Fatemeh Madan, Piret Arukuusk, Ly Pärnaste, Astrid Gräslund, Ülo Langel
Biochimica et Biophysica Acta

4 - Measurement of the Average Mass of Proteins Adsorbed to a Nanoparticle by Using a Suspended Microchannel Resonator Reza Nejadnik, Wim Jiskoot
Wiley Online Library

5 - Dynamic responses in small intestinal mucus: Relevance for the maintenance of an intact barrier Catherine Taylor Nordgård, Kurt I. Draget
European Journal of Pharmaceutics and Biopharmaceutics

6 - Steric and interactive barrier properties of intestinal mucus elucidated by particle diffusion and peptide permeation Marie Boegh, María García-Díaz, Anette Müllertz. Hanne Mørck Nielsen
European Journal of Pharmaceutics and Biopharmaceutics

7 & 7b - Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro Stine Rønholt, Mie Kristensen, Hanne Mørck Nielsen
(book) Cell-penetrating peptides: Methods and Protocols 2nd Edition, Springer

8 - Improved insulin loading in poly(lactic-co-glycolic) acid (PLGA) nanoparticles upon self-assembly with lipids María García-Díaz, Camilla Foged, Hanne Mørck Nielsen
International Journal of Pharmaceutics

9 - Bioactive protein-based nanofibers promotes transepithelial permeation of intact therapeutic 1 protein by interactions with biological components Karen Stephansen, María García-Díaz, Flemming Jessen, Ioannis S. Chronakis, Hanne Mørck Nielsen

10 - Abrasion of Adsorbed Protein is the Root Cause of Sub-Visible Particle Formation during Stirring A.S. Sediq, R.B. van Duijvenvoorde, W. Jiskoot, M.R. Nejadnik
Journal of Pharmaceutical Sciences

11 - Preclinical safety and efficacy models for pulmonary drug delivery of antimicrobials with focus on in vitro models Marius Hittinge, Jenny Juntke, Stephanie Kletting, Nicole Schneider-Daum, Cristiane de Souza Carvalho, Claus-Michael Lehr
Advanced Drug Delivery Reviews

12 - Overcoming the pulmonary barrier: new insights to improve the efficiency of inhaled therapeutics Xabier Murgia, Cristiane De Souza Carvalho, Claus-Michael Lehr
European Journal of Nanomedicine

13 - Overview of Inhaled Nanopharmaceuticals Sarah Barthold, Nicole Kunschke, Xabier Murgia, Brigitta Loretz, Cristiane De Souza Carvalho, Claus-Michael Lehr
ISAM Textbook of Aerosol Medicine, Chapter: 8.1, Publisher: International Society for Aerosols in Medicine, Editors: Barbara Rothen-Rutishauser and Rajiv Dhand,

14 - Biosimilar intestinal mucus protects Caco-2 epithelial monolayers from damage caused by fed state intestinal components Ditlev Birch, Philip C. Christophersen, Huiling Mu, Hanne M. Nielsen

15 - IgG-loaded hyaluronan-based dissolving microneedles for intradermal protein delivery Juha Mönkäre, M. Reza Nejadnik, Khalil Baccouche, Stefan Romeijn, Wim Jiskoot, Joke A. Bouwstra
Journal of Controlled Release

16 - Conjugation of Cell-Penetrating Peptides to Parathyroid Hormone Affects Its Structure, Potency, and Transepithelial Permeation Mie Kristensen, Anne Marit de Groot, Jens Berthelsen, Henrik Franzyk, Alice Sijts, Hanne Mørck Nielsen
Bioconjugate Chemistry

17 - Liposome functionalization with copper-free “click chemistry” Erik Oude Blenkea, Gruson Klaasseb, Hannes Mertenc, Andreas Plückthunc, Enrico Mastrobattistaa, Nathaniel I. Martinb
Journal of Controlled Release

18 - Nucleic Acid Therapeutics Using Polyplexes: A Journey of 50 Years (and Beyond) Ulrich Lächelt, Ernst Wagner
Chemical Reviews

19 - Complexity in the therapeutic delivery of RNAi medicines: an analytical challenge Stefano Colombo, Xianghui Zeng, Heloise Ragelle, Camilla Foged
Expert Opinion

20 - Mechanistic profiling of the siRNA delivery dynamics of lipid-polymer hybrid nanoparticles Colombo, S.; Cun, D.; Remaut, K.; Bunker, M.; Zhang, J.; Martin-Bertelsen, B.; Yaghmur, A.; Braeckmans, K.; Nielsen, H.M.; Foged, C.
J. Control. Release 201, 22-31

21 - A convergent uptake route for peptide- and polymer-based nucleotide delivery systems Lindberg, Staffan; Regberg, Jakob; Eriksson, Jonas; Helmfors, Henrik; Munoz-Alarcon, Andres; Srimanee, Artita; Figueroa, Ricardo A.; Hallberg, Einar; Ezzat, Kariem; Lange, Ulo
J. Control. Release

22 - Peptide-Based Delivery of Oligonucleotides Across Blood-Brain Barrier Model Srimanee, Artita; Regberg, Jakob; Hallbrink, Mattias; Kurrikoff, Kaido; Veiman, Kadi-Liis; Vajragupta, Opa; Langel, Ulo
International journal of peptide research and therapeutics

23 - Rational design of a series of novel amphipathic cell-penetrating peptides Regberg, Jakob; Srimanee, Artita; Erlandsson, Mikael; Sillard, Rannar; Dobchev, Dimitar A.; Karelson, Mati; Langel, Ulo
International Journal of Pharmaceutics

24 - Efficient cell-specific uptake of binding proteins into the cytoplasm through engineered modular transport systems Wouter P.R. Verdurmen, Manuel Luginbühl, Annemarie Honegger, Andreas Plückthun
J. Control. Release

25 - Surface coating of siRNA-peptidomimetic nano-self-assemblies with anionic lipid bilayers: Enhanced gene silencing and reduced adverse effects in vitro Xianghui Zeng, Anne Marit de Groot, Alice J.A.M. Sijts, Femke Broere, Erik Oude Blenke, Stefano Colombo, Willem van Eden, Henrik Franzyk, Hanne Mørck, Camilla Foged
Royal Society of Chemistry

26 - Brain delivery research in public-private partnerships: The IMI-JU COMPACT consortium as an example. Axel H. Meyer, Christopher Untucht, Georg C. Terstappen

27 - BBB endothelial transcytosis research and perspectives Imre Mäger, Axel H. Meyer, Matthew J.A. Wood

28 - Targeting bbb transcytosis for brain drug delivery Imre Mäger, Axel H. Meyer, Matthew J.A. Wood

29 - Targeted siRNA Delivery Using a Cationic Lipo-Oligoaminoamide Core and a Transferrin Protein and Influenza Peptide Shell (Not directly funded by COMPACT) Wei Zhang, Katharina Müller, Eva Kessel, Sören Reinhard, Dongsheng He, Philipp M. Klein, Miriam Höhn, Wolfgang Rödl, Ernst_Wagner
Advanced Healthcare Materials

30 - Coiled coil interactions for the targeting of liposomes for nucleic acid delivery Erik Oude Blenke, Joep van den Dikkenberg, Bartjan van Kolck, Alexander Kros, Enrico Mastrobattista
Royal Society of Chemistry

31 - pH-responsive PepFect cell-penetrating peptides Jakob Regberg, Luis Vasconcelos, Fatemeh Madani, Ülo Langel, Mattias Hällbrink

32 - Role of scavenger receptors in peptide-based delivery of plasmid DNA across a blood–brain barrier model Artita Srimaneea, Jakob Regbergb, Mattias Hällbrinkb, Opa Vajraguptad, Ülo Langel
International Journal of Pharmaceutics.

33 - Quantitative structure–activity relationship refinement improves efficacy of generation of novel cell-penetrating peptides Moataz Dowaidara, Jakob Regberga, Dimitar A. Dobchevc, Tõnis Lehtoa, Mattias Hällbrink, Mati Karelsonb, Ülo Langela

34 - Determination of the activity of maleimide-functionalized phospholipids during preparation of liposomes Mira Oswald, Simon Geissler, Achim Goepferich
International Journal of Pharmaceutics

35 - Co-culture of human alveolar epithelial (hAELVi) and macrophage (THP-1) cell lines Stephanie Kletting, Sarah Barthold, Urska Repnik, Nicole Schneider-Daum, Gareth Griffiths, Brigitta Loretz, Cristiane de Souza Carvalho-Wodarz, Claus-Michael Lehr

36 - How to Tackle the Challenge of siRNA Delivery with Sequence-Defined Oligoamino Amides Sören Reinhard, Ernst Wagner
Macromolecular Bioscience

37 - Preparation of nanosized coacervates of positive and negative starch derivatives intended for pulmonary delivery of proteins Sarah Barthold, Stephanie Kletting, Julian Taffner, Cristiane de Souza Carvalho-Wodarz, Elise Lepeltier, Brigitta Loretz, Claus-Michael Lehr
Journal of Materials Chemistry B

38 - Human alveolar epithelial cells expressing tight junctions to model the air-blood barrier Anna Kuehn, Stephanie Kletting, Cristiane de Souza Carvalho-Wodarz Urska Repnik, Gareth Griffiths, Ulrike Fischer, Eckart Meese, Hanno Huwer, Dagmar Wirth, Tobias May, Nicole Schneider-Daum, Claus-Michael Lehr

39 - The effect of nanoparticles on airway mucus functional properties, a case study Catherine Taylor Nordgård, Kurt I. Draget
Annual transactions of the nordic rheology society,

40 - Multimodal fluorescence- and bioluminescence imaging reveals transfection potential of intratracheally administered polyplexes for breast cancer lung metastases Antonia Geyer, Alexander Taschauer, Fatih Alioglu, Martina Anton, Julia Maier, Elisabeth Drothler, Manuela Simlinger, Sümeyye Yavuz, Haider Sami, Manfred Ogris1

41 - Fluorescence Labelling of Extracellular vesicles Using a Novel Thiol-Based Strategy for Quantitative Analysis of Cellular Delivery and Intracellular Traffic. Cocks Alex., Falcon-Perez, Juan, Sayers Edd, Webber Jason, Watson Peter., Clayton Aled., Jones Arwyn
Nanoscale 9

42 - Role of autophagy in cell-penetrating peptide transfection model Dowaidar M, Gestin M, Cerrato CP, Jafferali MH, Margus H, Kivistik PA, Ezzat K, Hallberg E, Pooga M, Hällbrink M, Langel Ü
Sci Rep. 2017 Oct 3;7(1):12635

43 - Simultaneous membrane interaction of amphipathic peptide monomers, self-aggregates and cargo complexes detected by fluorescence correlation spectroscopy. Vasconcelos L, Lehto T, Madani F, Radoi V, Hällbrink M, Vukojević V, Langel Ü.
Biochim Biophys Acta. 2017 Sep 26. pii

44 - Assisted Self-assembly of Cell Penetrating Peptides-Oligonucleotides Complexes for Gene Delivery Dowaidar M, Abdelhamid HN, Hällbrink M, Freimann K, Kurrikoff K, Zou, X, Langel
Sci Rep.

45 - Graphene oxide nanosheets in complex with cell penetrating peptides for oligonucleotides delivery Dowaidar M, Abdelhamid HN, Hällbrink M, Zou X, Langel U
Biochim Biophys Acta. 2017 Sep;1861(9):2334-2341

46 - Saturated Fatty Acid Analogues of Cell-Penetrating Peptide PepFect14: Role of Fatty Acid Modification in Complexation and Delivery of Splice-Correcting Oligonucleotides Lehto T, Vasconcelos L, Margus H, Figueroa R, Pooga M, Hällbrink M,Langel U.
Bioconjug Chem. 2017 Mar 15;28(3):782-792.

47 - Targeting the Central Nervous System (CNS): A Review of Rabies Virus-Targeting Strategies Simon Geissler; Achim Goepferich
Molecular Pharmaceutics

48 - HPLC analysis as a tool for assessing targeted liposome composition Michael Platscher, Simon Geissler; Achim Goepferich
International Journal of Pharmaceutics

49 - Endosomal Size and Membrane Leakiness Influence Proton Sponge-Based Rupture of Endosomal Vesicles Lotte M.P. Vermeulen, Toon Brans, Sangram K. Samal, Peter Dubruel, Jo Demeester, Stefaan C. De Smedt, Katrien Remaut, Kevin Braeckmans
submitted to ACS Nano

50 - A quantitative comparison of cytosolic delivery via different protein uptake systems Verdurmen W.P.R., Mazlami M. Plückthun R.
Sci Rep. 7

51 - A biotin ligase-based assay for the quantification of the cytosolic delivery of therapeutic proteins Verdurmen W.P.R., Mazlami M. Plückthun R.
Methods Mol. Biol. 1575

52 - Intradermal vaccination with hollow microneedles: A comparative study of various protein antigen and adjuvant encapsulated nanoparticles Guangsheng Du, Rania M. Hathout, Maha Nasr, M. Reza Nejadnik, Jing Tu, Roman I. Koning, Abraham J. Koster, Bram Slütter, Alexander Kros, Wim Jiskoot, Joke A. Bouwstra, Juha Mönkäre
Journal of Controlled Release

53 - PLGA nanoparticle loaded dissolving microneedles compared to hollow microneedles in dermal vaccine delivery Juha Mönkäre, Maria Pontier, Guangsheng Du, Mara Leone, Reza M. Nejadnik, Conor O’Mahony, Bram Slütter, Wim Jiskoot, Joke A. Bouwstra
To be determined, manuscript in preparation

54 - Barriers and motivations for non-invasive drug delivery Brigitta Loretz, Nicole Schneider-Daum, Maike Windbergs, Ulrich Schaefer, Marc Schneider, Claus-Michael Lehr
European Journal of Pharmaceutics and Biopharmaceutics

55 - Fluorescence- and computed tomography for assessing the biodistribution of siRNA after intratracheal application in mice Antonia Geyer, Cornelia Lorenzer, Sebastian Gehrig, M., Simlinger Johannes Winkler, Haider Sami, Manfred Ogris
International Journal of Pharmaceutics

56 - Engineering of small interfering RNA-loaded lipidoid-poly(DL-lactic-co-glycolic acid) hybrid nanoparticles for highly efficient and safe gene silencing: A quality by design-based approach Kaushik Thanki, Xianghui. Zeng, S.Justesen, S.Tejlmann, E. Falkenberg, E. Van Driessche, Hanne Mørck Nielsen, Henrik Franzyk, Camila Foged,
European Journal of Pharmaceutics and Biopharmaceutics

57 - Functional Delivery of Lipid-Conjugated siRNA by Extracellular Vesicles Aisling J. O’Loughlin,1 Imre Mäger,1,2 Olivier G. de Jong,1 Miguel A. Varela,1 Raymond M. Schiffelers,3 Samir El Andaloussi,1,4 Matthew J.A. Wood,1 and Pieter Vader1,3
Molecular Therapy

58 - Ultrafiltration with size-exclusion liquid chromatography for high yield isolation of extracellular vesicles preserving intact biophysical and functional properties Joel Z. Nordin, M.D.a,1, Yi Lee, BScb,1, Pieter Vader, PhDb, Imre Mäger, PhDb,c, Henrik J. Johansson, PhDd, Wolf Heusermann, PhDe, Oscar P.B. Wiklander, M.D.a, Mattias Hällbrink, PhDf, Yiqi Seow, DPhilg, Jarred J. Bultema, PhDh, Jonathan Gilthorpe, PhDi, Tim Davies, MScj, Paul J. Fairchild, PhDj, Susanne Gabrielsson, PhDh, Nicole C. Meisner-Kober, PhDe, Janne Lehtiö, PhDd, C.I. Edvard Smith, M.D., PhDa, Matthew J.A. Wood, M.D., DPhilb,⁎, Samir EL Andaloussi, PhDa, b,⁎⁎

59 - Therapeutic Potential of Multipotent Mesenchymal Stromal Cells and Their Extracellular Vesicles Nina Heldring,1,* Imre Ma¨ ger,2,3 Matthew J.A. Wood,2 Katarina Le Blanc,1 and Samir E.L. Andaloussi1,2
Human Gene Therapy

60 - Extracellular vesicle in vivo biodistribution is determined by cell source, route of administration and targeting Oscar P. B. Wiklander1$, Joel Z. Nordin1$, Aisling O’Loughlin2, Ylva Gustafsson3, Giulia Corso1, Imre Ma¨ ger2,4, Pieter Vader2, Yi Lee2, Helena Sork1, Yiqi Seow5, Nina Heldring1, Lydia Alvarez-Erviti6, CI Edvard Smith1, Katarina Le Blanc1,7, Paolo Macchiarini3, Philipp Jungebluth3,8, Matthew J. A. Wood2 and Samir EL Andaloussi1,2*
Journal of extracellular vesicles

61 - Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles Jinghuan Li1§, Yi Lee1§, Henrik J. Johansson2, Imre Ma¨ ger1,3, Pieter Vader1,4, Joel Z. Nordin5, Oscar P. B. Wiklander5, Janne Lehtio¨ 2, Matthew J. A. Wood1* and Samir EL Andaloussi1,5
Journal of extracellular vesicles

62 - Cells release subpopulations of exosomes with distinct molecular and biological properties Eduard Willms1, Henrik J. Johansson2, Imre Mäger1,3, Yi Lee1, K. Emelie M. Blomberg4, Mariam Sadik5, Amr Alaarg1, C.I. Edvard Smith4, Janne Lehtiö2, Samir EL Andaloussi1,4, Matthew J.A. Wood1,* & Pieter Vader1,5,*
Scientific Reports

63 - Hollow microneedle-mediated intradermal delivery of model vaccine antigen-loaded PLGA nanoparticles elicits protective T cell-mediated immunity to an intracellular bacterium. Du G, Mönkäre J, Platteel ACM Broere F, Bouwstra JA, Sijts AJAM.
Journal of control release

64 - Intradermal vaccination with hollow microneedles: A comparative study of various protein antigen and adjuvant encapsulated nanoparticles. Hathout RM2, Nasr M2, Nejadnik MR1, Tu J3, Koning RI4, Koster AJ4, Slütter B5, Kros A3, Jiskoot W1, Bouwstra JA6, Mönkäre J1.
Journal of control release